The wave bioreactor system produces viruses under complete containment without the need for biosafety cabinet. Wave bioreactor 200 system ge healthcare life sciences. Each installed instrument has a set of default parameter values, called system settin. Readytoprocess wave 25 system handbook 29009598 ab. Recently, a number of manufacturingscale perfusion or perfusioncapable bioreactors have been launched table 2, and this includes the cur. Developed in response to customer demand, this unit combines four key instruments previously available as separate controllers in a. Ge healthcare ge wave ge wave bioreactor 210 2050 tags. Culture medium and cells contact only a presterile, disposable chamber that is positioned on a special rocking platform. Its modular system consists of a base unit and various integral options suitable for culture of multiple cell lines. An overview of bioreactors types, their design and properties will be considered for biomolecules production such as biomass, enzymes, organic acids, aroma compounds, spores, mushroom production. Read and understand this users guide before operating. Cart cell therapies have been successfully generated using the xuri ces w25 system, which reduces manual labor compared with static culturing methods. Furthermore, the volume in cellbag disposable bioreactors can be increased by a factor of 10. Wave bioreactor workflow for 150 l bioreactor standard construct for cip sequence ewis free format ewis.
Successfully protecting contents, the sealing bags permit tests to be accurately completed without. Using patented wave cell bags, the risk of cross contamination is eliminated, and the tray can be. These are products by a single manufacturer or a list of manufacturers. Triplicate cultures demonstrate a reproducible and robust batch production of adenovirus. Wave biotech lab instruments, equipment, and accessories. Integrated singleuse bioreactor and controller systems. Optimization of microcarrierbased virus production in single. Developed in response to customer demand, this unit combines these four key instruments, previously available as separate controllers, into a single, compact device.
For further information regarding the use of wave bioreactor 210, wave bioreactor 2050 and wavepod ii1, refer to wave bioreactor system base 210 eh operating instructions and wave bioreactor 2050. View online or download ge readytoprocess wave 25 operating instructions manual. Sterile sample bags are ideal for transporting and storing solids, semisolids, and liquids. Presterilized, singleuse cellbag bioreactors protect against the risk of crosscontamination, require no cleaning, and involve minimal validation and they are supplied in a readytouse format reliability.
Reactions happen within the enclosed, presterilized cell bag set on a temperaturecontrolled tray, that eliminates the chance of cross. The new wavepod instrument module from wave biotech integrates all instrumentation associated with the bioreactor, such as do, ph, and gas composition controls. Interchangeable holders can be replaced in seconds. Wave bioreactor 2050, wavepod, and external instrument modules, and their intended use. As changes in temperature affect the density of water, any deviation from the calibrated condition will affect instrument accuracy.
Developed in response to customer demand, this unit combines four key instruments previously available as separate controllers in a single portable device. Jan 16, 2019 culture of mammalian or human cells in wave bioreactor is widely used for cell expansion or for biologics manufacturing. Bioprocess automation software thermo fisher scientific us. My 14 years of experience in diagnostic field helped us to achieve our goals. The rocker is the main unit and is used together with a tray and a disposable cellbag bioreactor. Through innovation to quality, new concepts to increase productivity in laboratory fermentation and cell culture experiments. Because data has been collected from such a diverse assortment of sites from inside harbors and basins, from the pilings of piers and oil platforms, from isolated nearshore and remote offshore locations each sensor has to be carefully chosen and prepared for its specific environment. Part component description 1 uninet9port powerconnectiontotherocker. Enduser license agreement for readytoprocess wave 25 system. The amount of energy in the shockwaves is controlled with different acceleration of the projectile. Use it as basic system or in combination with the biostat b control tower and singleuse optical ph and do sensors to enable advanced control strategies for batch, fedbatch and continuous perfusion. Cytiva readytoprocess wave 25 rocking bioreactor system by cytiva. The wavepod connects via a digital link to a wave bioreactor base, and its. This manual provide you with the instructions needed to handle wave bioreactor system optional standalone instruments.
After requirements were established, the electronic workflow was approached in a bottomup fashion. An early pioneer in the area of disposable bioreactors, the wave bioreactor was the first singleuse bioreactor system when it was presented to the bioprocessing community in 1996. The ge wave bioreactor system 210eh offers users a digitally controlled, cost effective cell culturing instrument. Wave bioreactor 210 and 2050 systems pdf free download. Dasgip benchtop bioreactors feature an autoclavable glass body and a stainless steel head plate. The adjustable rocking platform tilts side to side in a gentle rocking motion, mixing and transferring oxygen into the culture medium. This chapter details how to initiate a culture, install the xuri ces w25, and install a 2 l cellbag bioreactor. This modular design consists of a base rocker and various integral options suitable for the culture of multiple cell lines.
Readytoprocess wave 25 cultures rocking at 7, 8, and 9 rpm6. Cdip has used a wide range of instrumentation over the years. Tendon and ligament engineering might provide an alternative tissue source for the surgical replacement of injured tendon. Please note any thermometers, gas supply tanks, regulators, or dataloggers seen in our photos is not included unless otherwise noted in the listing. Each installed instrument has a set of default parameter values, called system settings. Engineered for durability, it can be used in both research and development as well as production environments.
The ability to handle the bioreactor in a tilt position facilitates sampling and harvest. Readytoprocess wave 25 system handbook 29009598 ac. Rswt treatment point time pressure pressure pulses pulse tissue generator. Ge wave biotech bioreactor system 210eh 210 eh wwarranty. Documents document view page ge wave bioreactor 210. Valley instrument 503m temp control 3 available 503mcl001p1s. The rocking motion of the platform induces waves in the culture fluid and thereby provides continual mixing and oxygen transfer. The biostat rm basic rocking platform with an integrated local controller, air co2 mixing module and optional load cells is the optimal choice. Bioreactors wave biotech, llc pharmaceutical manufacturing. It ensures a very short mixing time and a high gas liquid mass transfer on a small scale. The future is now, when one considers an automated biosampling system. Multiple instrument configurations for suspension, microcarrier, batch. Biostat rm 20 50 basic is a perfectly sized, singleuse, wave mixed benchtop bioreactor for standalone use. A general understanding of bioreactor systems and their operation is important prior to using the system for the first time.
Designed for use with wave bioreactor systems, the cellbag bioreactors are presterilized, singleuse bags for noninvasive cell cultivation in research, development, and commercial manufacturing operations. The effective amount of energy in the tissue is in. The wave bioreactor instrument is an effective, costefficient device for cell culture. Ge healthcare wave bioreactor system 200eh operator manual important user. Wave bioreactor 210 and 2050 systems operating instructions aa 5. Operating mode with one cellbag bioreactor on the rocker. Specifications for wave bioreactor 210 and 2050 systems base 210eh base 2050eht. Culture medium and cells contact only a presterile, disposable chamber called a cellbag that is placed on a. Similar cell growth and cell concentrations were reached during the three processes. It is based on a wellknown rocking technology providing mixing and aeration to the culture, which is maintained in an inflated, disposable cellbag bioreactor cultivation chamber. Catch the wave a discussion on using the wave disposable. To select the correct wave bioreactor system for your application, first determine the cellbag bioreactor size you need by estimating the culture volume you require and then select the appropriate instrumentation and options table 1. The finesse g3lite bioreactor controller is a cartmounted, selfcontained unit that can be operated singly or networked.
Since then, the range of products has expanded to include larger scale systems and advanced optical sensor and control technologies. Cultivation of t cells in suspension using wave bioreactor systems 210 and 2050 cultivation of e. Ge wave biotech irc sterile tube fuser stfircww low weld use stfir w. Optimizing process control in wave bioreactor 2050 system. Wave bioreactor cultivation of sensitive cells such as stem cells, immune cells or anchoragedependent cells, is recognized as an attractive option for culture in suspension or adherently on microcarriers. Readytoprocess wave 25 bioreactor system this application note describes the production of adenovirus in hek293 suspension cells grown in hyclone cdm4hek293 culture medium using the readytoprocess wave 25 bioreactor system. The thermo scientific hyperforma singleuse bioreactor s.
Ge wave 2050eht bioreactor 50 liter tray with warranty. This particular culture conditions are mostly achieved in socalled high performance bioreactors. Wave bioreactor 210 system can be used with the following cellbag volumes. Readytoprocess wave 25 ge healthcare life sciences. The ergonomic rocker design makes handling convenient and easy. Highperformance software control platforms, enhancing both user experience and data aggregation. Study of hydrodynamics in wave bioreactors by computational. Wave biotech fullyautomated sterile tubing fuser aseptically connects both masterflex pump tubing and generalpurpose transfer tubing from 14 to 78 od. The g3lite bioreactor controller leverages the latest technologies, including singleuse sensors and finesse trubio software. For clinical settings, bioreactors also have the advantages of lesscontamination risk, high reproducibility of cell propagation by minimizing manual operation, and a.
Wave bioreactor system 200eh ge healthcare life sciences. Ge wave cell bag bioreactor with wavepod ii is available from the lab world group. It features an exchangeable bag holder to fit bags with a total volume of 1 50 l. Pdf evaluation of a novel wave bioreactorr cellbag. Standalone instruments for wave bioreactor 210 and 2050. The wave bioreactor system was the first singleuse bioreactor on the market when it was introduced in the late 1990s. High quality and innovative lab scale fermentor and benchtop bioreactor at a reasonably low cost. The system is a cell culture instrument for the production of recombinant proteins in mammalian and insect cell lines in batch, fedbatch, and perfusion culture. Wave bioreactor systems 210 and 2050 ge healthcare.
General electric systems technology manual chapter 3. Wave bioreactor 2050 system the wave bioreactor 2050 system fig 4 is modular, consisting of an electrically powered base unit base2050eht and a choice of kits for holding the cellbag bioreactor. New chemcell modular benchtop bioreactor system consists of a heavyduty base unit and a choice of stainless steel rocker trays. The thermo scientific hyperforma rocker bioreactor is controlled by the hyperforma g3lab controller and trubio software, providing a complete, readytouse solution. Culture medium and cells contact only a presterile, disposable chamber that is positioned on a special rocki. Cart cell expansion in a xuri cell expansion system w25. Ge readytoprocess wave 25 operating instructions manual pdf. Documents document view page ge wave bioreactor 210 20. There was no significant difference in growth rate between cytodex 1 and cytodex 1 gamma cultures or between using wave bioreactor 2050 and readytoprocess wave 25 systems. Bioreactors instrumentation and control flow measurement. Cell culture was conducted in cellbag bioreactors manufactured from either bioclear 11 or fortem films. And control a wellstirred tank, one of the most commonly employed bioreactors, approximates reasonably well to the idealized state of perfect mixing. Protocols on inoculation, monitoring, and sampling are also outlined in this chapter.
Ge wave cell bag bioreactor with wavepod ii youtube. Our proven wave mixed bioreactor consists of a rocker unit with bag holder and pillowshaped flexsafe rm singleuse bags for culture volumes from 100 ml to 25 l. Jul 08, 20 an early pioneer in the area of disposable bioreactors, the wave bioreactor was the first singleuse bioreactor system when it was presented to the bioprocessing community in 1996. The controller is engineered to optimize capital costs without sacrifi cing functionality. The first step was creating the basic building blocks, which are called instructions or manual phases.
System specifications and selection to select the correct wave bioreactor system for your application, first determine the cellbag bioreactor size you need by estimating the culture volume you require and then select the appropriate instrumentation and options table 1. The wave bag bioreactor 2050eh is a flexible design built ideal for cell culturing, protein manufacturing, cho cells, vaccination production along with various applications. This is our testing tools to show buyers the instrument is working properly up to manufacturer specification and has been tested by our. Pdf application of different types of bioreactors in. The high oxygen supply capability of the wave bioreactor system makes it ideal for insect cell culture. Adenovirus production in singleuse readytoprocess wave. There is a myriad of equipment hardware choices to be made. A bioreactor provides a controllable environment enabling the systematic stud tendon and ligament injury is a worldwide health problem, but the treatment options remain limited. Biostat b benchtop bioreactor controller sartorius. Adenovirus production in singleuse readytoprocess wave 25. Introduction adenovirus is one of the most frequently used viral vectors in. Furthermore, the tendonligament bioreactor system can provide a suitable culture environment, which mimics the dynamics of the in vivo environment for tendonligament maturation. Wave bioreactor 2050 system with wavepod ii integrated controller. The rocking motion of the platform induces waves in the culture fluid and thereby provides continual mixing and oxygen transfer, resulting in a robust environment for cell growth.
The wide top opening facilitates for the user to interact with the culture without removing the lid. Benefit from our excellent global application support and technical service and get started right away. Readytoprocess wave 25 system components the readytoprocess wave 25 system consists of a rocker, cbcu gas mixers, and pumps, all operated by the unicorn software installed on a client computer. Wave bioreactor system 2050eh wave bioreactor 2050 system rocking base the rocking base is electrically heated and it is designed to inflate and rock the bioreactor for rapid gas transfer and mixing. Wave bioreactor systems produce a very lowshear environment while maintaining mixing and oxygenation, they are ideal for the use of microcarriers. Bioreactor system base 210 eh operating instructions and wave bioreactor 2050 and wavepod ii user manual, respectively.
Like other xcellerex singleuse bioreactor systems, this model is suitable for cgmp clinical and commercial manufacture of biotherapeutics, vaccines, and other biologics. Biostat rm and flexsafe rm for seed production and small. All parts are laserlabelled with part numbers and have certificates of origin. Cellbag bioreactors, including all fittings and filters, are supplied. The first step was creating the basic building blocks, which are called instructions or manual. User manual wave bioreactor 210 and 2050 systems manualzz. Ge healthcare 28988000 wave25 readytoprocess rocker. The system, comprising a rocker with a builtin co2 mixer and a singleuse cellbag bioreactor cell culture chamber, was an immediate success. Thermo scientific trubio, truchrom, and trupur automation software powered on the deltav distributed control platform from emerson facilitate easy integration of multiple vessels to streamline process control and data management from research to production scale applicationscombining robust. The biostat rm, a fully gmp compliant, singleuse, wave mixed bioreactor and flexsafe bags are proven for a broad range of different cell lines incl. Cls1200 chemcell rocker bioreactor system, for cell.
Wave bioreactor systems 210 and 2050 ge healthcare life. Ge wave bioreactor system 20 50 eht the lab world group. The singleuse readytoprocess wave 25 bioreactor system is a reliable and intuitive cell culture device for working volumes up to 25 l. The readytoprocess wave 25 rocker features integrated temperature sensors and load cells, designed for convenient handling and control of cell cultures up to 25 l. System200eh, co2,o2, analog cytiva, formerly ge healthcare. The wave 2050eht bioreactor has a long line of instrumentation such as dissolved oxygen, amplifier, and weight controllers for perfusion culture and ph control.
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